A standard curve is required for each DiaPharma Factor X kit. Five standards must be included in each test run. For standardization, use commercially supplied hemostasis calibration plasma, or a pool of calibrated fresh frozen normal plasma obtained in the same manner as the plasma to be tested.
Preparation of Standards
| % FX | Normal Plasma, mL | Buffer, mL |
| 100 | 100 | – |
| 75 | 75 | 25 |
| 50 | 50 | 50 |
| 25 | 25 | 75 |
| 10 | 10 | 90 |