- Bilirubin, hemoglobin, and plasma from hyperlipemic patients give difficulties in absorbance readings. In these cases individual plasma blanks are necessary.
- Heparin concentrations below 30 U/ml are neutralized by Polybrene® and do not influence the assay.
- Temperature control and incubation times are critical.
- Proper mixing is important to be sure that the reaction mixture is homogenous, but avoid vigorously shaking, as the proteins may precipitate in the foam.